< Previous28 Superior Reliability & Performance OPTICAL OUTPUT AChameleon Discovery NX with Total Power Control Tuning Range (nm)660 to 1320 Average Output Power (mW) 700 nm 800 nm 900 nm 1000 nm 1200 nm 1300 nm 1250 2200 2100 1600 1300 1075 Pulse Duration 1,2 (fs)100 Repetition Rate (MHz)80 ±0.5 Beam Mode 1 M 2 <1.2 Beam Diameter 1 (mm)1.2 ±0.2 Ellipticity 1 0.8 to 1.2 Astigmatism 1 (%)<25 PolarizationLinear, Horizontal Noise 1,3 (%)<0.5 Power Stability 4 (%)±1 Tuning Speed 5 (nm/s)>50 Pointing Accuracy 6 (µrad)<350 Rise/Fall Time (ns)<1000 Contrast Ratio1000:1 Dispersion Compensation Range (fs 2 ) 680 nm 800 nm 950 nm 1050 nm 1300 nm 0 to -40,000 0 to -17,000 0 to -9000 0 to -5000 0 to -4000 OPTICAL OUTPUT B (Optional) Wavelength (nm)1040 Average Output Power (mW)>2800 Pulse Duration 2 (fs)140 Repetition Rate 7 (MHz)80 ±0.5 Beam ModeM 2 <1.2 Beam Diameter (mm)1.2 ±0.2 Ellipticity0.8 to 1.2 Astigmatism (%)<25 PolarizationLinear, Horizontal Noise 3 (%)<0.25 Power Stability 4 (%)±1 Rise/Fall Time (ns) <1000 Contrast Ratio1000:1 Oscillators Chameleon Discovery NX with Total Power Control Ultrafast Laser Systems 1 At 900 nm. 2 Assumes sech2 pulse shape. 3 RMS, 10 Hz to 10 MHz. 4 Power drift in a 2 hour period after 1 hour warm-up and ±1°C ambient temperature change. 5 Averaged over entire tuning range. 6 Maximum deviation over entire GDD dispersion adjustment and wavelength range. 7 Phase locked to Output A.29 coherent.com Oscillators Ultrafast Laser Systems Chameleon Discovery NX with Total Power Control UTILITY REQUIREMENTSChameleon Discovery/Discovery NX with Total Power Control Operating Voltage (VAC)90 to 250 (auto ranging) Maximum Operating Current (A) Power Supply Chiller MRU <8 at 90 VAC <14 at 90 VAC <2 at 90 VAC System Power Consumption (W)2300 Line Frequency (Hz)47 to 63 Communications/Control Interfaces 1 RS-232, USB, PC required (Analog in for TPC) ENVIRONMENTAL REQUIREMENTS Operating Temperature Range15 to 35°C (59 to 95°F) Storage Temperature Range0 to 40°C (32 to 104°F) HumidityNon-condensing Altitude (m)<2000 MECHANICAL SPECIFICATIONS Power Supply Chiller MRU 19” unit, 3U 19” unit, 6U 19” unit, 2U 1 PC required.30 Superior Reliability & Performance Chameleon Ultra Ultrafast Laser Systems Chameleon Ultra Integrated, Automated and Widely Tunable Ti:Sapphire Oscillator More Chameleon Ultra systems are used for Multi-Photon Excitation (MPE) Microscopy and other non-linear imaging techniques than any other laser. Chameleon Ultra II delivers the highest power, the widest tuning range, the fastest tuning speed and the lowest noise of all commercially available Ti:Sapphire lasers. Its beam quality and pulse duration are specified to perfectly match your microscope or any other femtosecond experiment. Because of these capabilities, it is possible to image more dyes and fluorescent proteins, and to obtain deeper images more quickly than with any other laser. For example, the Chameleon Ultra II’s 680 nm to 1080 nm tuning range allows peak excitation of any marker – from short-wavelength dyes such as Indo or Fura, to new proteins like mCherry – as well as optimum native fluorescence excitation or SHG imaging. Every laser in the Chameleon Ultra family is compatible and has been tested with every inverted or upright MPE microscope on the market. The high power from the Chameleon Ultra II makes it an ideal pump source for the Chameleon Compact OPO wavelength extension, enabling imaging even further into the infrared. Proprietary PowerTrack and PermAlign technologies ensure stable performance over the laser lifetime without any adjustments. The features of Chameleon Ultra combine to provide high throughput and quiet, reliable operation for thousands of hours, image after image. A broad range of accessories, including units for pre- compensation, harmonics generation, and OPO wavelength extension, enable more advanced studies where adjustable pulse duration and UV, visible or longer IR wavelengths are desirable. Oscillators Chameleon Ultra II Tuning Curve (typical) Wavelength (nm) Power (W) 0 4.5 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 650 Wavelength (nm) 70075080085090095010001050110031 coherent.com KEY ADVANTAGES • Highest power for deepest imaging • Automated tuning and alignment for hands-free operation • PowerTrack™ active alignment for long-term stability and low maintenance • Widest tuning range for efficient excitation of the widest gamut of probes • Simple menu-driven GUI or RS-232 for flexible, intuitive control • On-board spectrometer for real time spectral feedback • Extendable wavelength range from 340 nm to 4000 nm with Compact OPO Ultrafast Laser Systems Chameleon Ultra Oscillators SYSTEM SPECIFICATIONSChameleon UltraChameleon Ultra IChameleon Ultra II Average Power 1 (W)>2.5>2.9>3.5 Tuning Range (nm)690 to 1020690 to 1040680 to 1080 Peak Power 1 (kW)>200>250>300 Power Specifications >500 mW at 690 nm >1.4 W at 710 nm >2.5 W at 800 nm >1.4 W at 920 nm >450 mW at 1020 nm >600 mW at 690 nm >1.5 W at 710 nm >2.9 W at 800 nm >1.45 W at 920 nm >450 mW at 1020 nm >300 mW at 1040 nm >650 mW at 680 nm >1.6 W at 700 nm >3.5 W at 800 nm >1.6 W at 920 nm >550 mW at 1020 nm >200 mW at 1080 nm Tuning Speed 2 (nm/s)>35>40>40 Pulse Width 1,3 (fs)140 Noise 1,4 (%)<0.15 Output Power Stability 5 (%)<±0.5 Spatial Mode 1 TEM 00 (M 2 <1.1) Beam Diameter 1,6 (mm)1.2 ±0.2 Beam Ellipticity 1,7 0.9 to 1.1 Astigmatism 1 <10% Repetition Rate (MHz)80 PolarizationHorizontal >500:1 Pointing (μrad/nm)<0.5 Laser Head Physical Dimensions65.1 x 37.0 x 19.0 cm (25.64 x 14.53 x 7.45 in.) 1 Specified at peak of tuning range. 2 Average speed measured over entire tuning range. 3 Based on sech2 deconvolution of 0.65 times autocorrelation width. 4 Measured RMS in a 10 Hz to 20 MHz bandwidth. 5 Power drift in any two-hour period with less than ±1°C temperature change after a one-hour warm-up. 6 1/e2 at exit port. 7 Ratio of major to minor 1/e2 beam diameter at exit port.32 Superior Reliability & Performance Chameleon Vision and Vision-S Ultrafast Laser Systems Chameleon Vision and Vision-S Integrated, Automated and Widely Tunable Ti:Sapphire Oscillators The Chameleon Vision family has been designed to pre-com- pensate (pre-chirp) for the natural dispersion that occurs when femtosecond pulses pass through optical elements such as lenses. In some imaging applications, it may be useful to excite the sample with short pulses in order to maximize the efficiency of the non-linear process (two-photon excitation or harmon- ic generation). To satisfy this need, Chameleon Vision I and Vision II use the same ultrafast cavity design of the Chame- leon Ultra family, with the addition of a built-in automated, pre-compensation unit. As a result, the pulse duration at the sample can always be optimized to match its time-bandwidth limited value (about 140 fs at 800 nm). Chameleon Vision-S has been designed for those users who, in addition to pre-compensation, also want to excite the sample with even shorter pulses, but still retain the widest possible tuning range. Short pulses maximize the ratio between peak power and average power, an advantage for those samples where linear (average power-based) damage dominates over non-linear (peak power-based) damage. Chameleon Vision-S fully satisfies this requirement by provid- ing 75 fs pulses at the sample. Built-in pre-compensation supplies also an easy way to pur- posely change the pulse duration at the sample, to study of the interplay of peak power, average power and pulse dura- tion on different samples. For example, by mismatching the Chameleon Vision II Tuning Curve (typical) Chameleon Vision Maximum Negative Dispersion Capability Oscillators Wavelength (nm) Power (W) 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 650 Wavelength (nm) 700750800850900950100010501100 Wavelength (nm) GDD (fs 2 ) 0 50,000 40,000 30,000 20,000 10,000 650 Wavelength (nm) 700750800 The shaded region represents the range of dispersion values obtainable from the Vision 85090095010001050110033 coherent.com Ultrafast Laser Systems Chameleon Vision and Vision-S pre-compensation, it is possible to vary the pulse duration at the sample between the fully compensated value of 75 fs to chirped pulse durations of several hundreds of femtoseconds. All Chameleon Vision models incorporate our proprietary prism-based design, able to compensate the broadest range of GVD (Group Velocity Dispersion) using the smallest system footprint commercially available. The provided GVD compen- sation range is wide enough to eliminate the pulse broaden- ing associated with the most sophisticated microscopes, like ones including complex objective lenses, modulators and other optical elements. Chameleon Vision I and II deliver the highest average power, the broadest tuning range, the fastest tuning speed and the lowest noise available on the market among Ti:Sapphire lasers with pre-compensation. Chame- leon Vision S delivers the highest peak power at the sample, providing at the same time the broadest tuning range among auto-mated short pulse Ti:Sapphire lasers designed for non-linear imaging. This exceptional performance enables Chameleon Vision to easily manage pulse duration and excitation wavelength, providing brighter images and better sample viability. The broadly adjustable GVD compensation can be used to optimize image brightness even while pumping the OPO. The flexibility of the Vision family makes it ideal not only for microscopy but also for time-resolved spectroscopy and Terahertz studies. Chameleon Vision-S Peak Power (typical) Chameleon Vision-S Maximum Negative Dispersion Capability Oscillators Wavelength (nm) Negative Dispersion (fs 2 ) 0 50,000 40,000 30,000 20,000 10,000 Wavelength (nm) 70075080085090095010001050 Wavelength (nm) 65070075080085090095010001050 0 100 200 300 400 500 600 Peak Power (kW) KEY ADVANTAGES • Sealed construction for hands-free operation • Choice of 140 fs and 75 fs pulses • Automated pre-compensation with user-adjustable tables • Highest average output power among pre-chirped Ti:Sapphire lasers (Vision II) • Widest Ti:Sapphire tuning range available: 400 nm (Vision II) • Wide range of dispersion pre-compensation (0 to 47,000 fs2) • Simple menu-drive GUI or RS-232 operator interface for laser and pre-chirp management • On board spectrometer with USB interface for wavelength and bandwidth monitoring • PowerTrack active alignment and PermAlign manufacturing for long-term stability34 Superior Reliability & Performance Chameleon Vision and Vision-S Ultrafast Laser Systems Oscillators SYSTEM SPECIFICATIONSChameleon Vision IChameleon Vision II Tuning Range (nm)690 to 1040680 to 1080 Average Power at Peak (W)2.53.0 Power Specifications- 640 mW at 690 nm 1.07 W at 710 nm 2.5 W at 800 nm 920 mW at 920 nm 260 mW at 1040 nm - 500 mW at 680 nm - 1.5 W at 710 nm 3.0 W at 800 nm 1.35 W at 920 nm 400 mW at 1040 nm 180 mW at 1080 nm Dispersion Compensation Range 680 nm 690 nm 800 nm 1020 nm 1080 nm - 0 to 43,000 fs 2 0 to 22,000 fs 2 0 to 10,000 fs 2 - 0 to 47,000 fs 2 - 0 to 22,000 fs 2 0 to 10,000 fs 2 0 to 9000 fs 2 Tuning Speed 2 (nm/s)>35>40 Pulse Width 1,3 (fs)140 Noise 1,4 (%)<0.15 Output Power Stability 1,5 <±0.5 Spatial Mode 1 TEM 00 (M 2 <1.1) Beam Diameter 1,6 (mm)1.2 ±0.2 Beam Ellipticity 1.7 0.9 to 1.1 Astigmatism 1 (%)<10 Repetition Rate (MHz)80 PolarizationHorizontal >500:1 Pointing 8 (μrad/nm)<80/100 Operating Voltage (VAC)90 to 250 (auto ranging) Maximum Operating Current (A) Power Supply Chiller MRU x1 <15 at 90 VAC <7 at 90 VAC <2 at 90 VAC System Power Consumption (W)2300 max., 1300 typical Line Frequency (Hz)47 to 63 Operating Temperature Range15 to 35°C (59 to 95°F) Non-operating Temperature Range5 to 40°C (41 to 104°F) Weight of Laser Head52 kg (115 lbs.) Weight of Power Supply41 kg (90 lbs.) Umbilical Length3 m (10 ft.) Chiller: Dimensions (L x W x H) Weight 436 x 270 x 393 mm (17.17 x 10.63 x 15.47 in.) 11 kg (25 lbs.) MRU Air Recirculator: Dimensions (L x W x H) Weight 46 x 43 x 8.5 cm (18 x 17 x 3 in.) 9 kg (20 lbs.) 1 Specified at peak of tuning range. 2 Average speed measured over entire tuning range. 3 Based on sech2 deconvolution of 0.65 times autocorrelation width. 4 Measured RMS in a 10 Hz to 20 MHz bandwidth. 5 Power drift in any two-hour period with less than ±1°C temperature change after a one-hour warm-up. 6 1/e2 at exit port. 7 Ratio of major to minor 1/e2 beam diameter at exit port. 8 Measured over the whole wavelength and GDD dispersion adjustment range.35 coherent.com Ultrafast Laser Systems Chameleon Vision and Vision-S Oscillators SYSTEM SPECIFICATIONSChameleon Vision-S Tuning Range (nm)690 to 1050 Average Power at Peak (W) >2.5 Peak Power at Peak (kW)>440 Peak Power Specifications 80 kW at 690 nm 440 kW at 800 nm 240 kW at 920 nm 50 kW at 1050 nm Dispersion Compensation Range690 nm 0 to -43,000 fs 2 800 nm 0 to -22,000 fs 2 1050 nm 0 to -9500 fs 2 Tuning Speed 2 (nm/s) >25 Pulse Width 1,3 (fs)75 Noise 1,4 (%) <0.15 Output Power Stability 1,5 <±0.5 Spatial Mode 1 TEM 00 (M 2 <1.1) Beam Diameter 1,6 (mm) 1.2 ±0.2 Beam Ellipticity 1,7 0.9 to 1.1 Astigmatism 1 (%) <10 Repetition Rate (MHz) 80 PolarizationHorizontal >500:1 Pointing 8 (μrad/nm) <80/100 ENVIRONMENTAL SPECIFICATIONS Operating Temperature Range 15 to 28°C (59 to 82.5°F) Non-operating Temperature Range 5 to 40°C (41 to 104°F) Storage Temperature Range 5 to 40°C (41 to 104°F) ELECTRICAL REQUIREMENTS Operating Voltage (VAC)90 to 250 (auto ranging) Maximum Operating Current (A)<15 at 90 VAC (power supply) <7 at 90 VAC (chiller) <2 at 90 VAC (MRU) System Power Consumption (W) 2300 max., 1300 typical Line Frequency (Hz) 47 to 63 MECHANICAL PARAMETERS Weight of Laser Head 52 kg (115 lbs.) Weight of Power Supply 33 kg (73 lbs.) Umbilical Length 3 m (10 ft.) Dimensions (L x W x H) Chiller MRU Air Recirculator 436 x 270 x 393 mm (17.17 x 10.63 x 15.47 in.) 46 x 43 x 8.5 cm (18 x 17 x 3 in.) Weight Chiller MRU Air Recirculator 11 kg (25 lbs.) 9 kg (20 lbs.) 1 At 800 nm 2 Average speed measured over entire tuning range. 3 Based on sech 2 deconvolution of 0.65 times autocorrelation width. 4 Measured RMS in a 10 Hz to 20 MHz bandwidth. 5 Power drift in any two-hour period with less than ±1°C temperature change after a one-hour warm-up. 6 1/e 2 at exit port. 7 Ratio of major to minor 1/e2 beam diameter at exit port. 8 Measured over the whole wavelength and GDD dispersion adjustment range.36 Superior Reliability & Performance Chameleon Compact OPO and OPO-Vis Ultrafast Laser Systems Chameleon Compact OPO and OPO-Vis Synchronously-Pumped Optical Parametric Oscillator Accessories Chameleon Compact OPO is a fully automated Optical Para- metric Oscillator tunable between 1000 nm and 1600 nm. Chameleon Compact OPO-Vis, in addition to all the benefits of the Compact OPO, includes also Second Harmonic Gen- eration (SHG) outputs of the Chameleon and the OPO signal wavelengths to provide additional seamless tuning between 340 and 800 nm. Designed specifically to be pumped by the Chameleon Ultra and Vision families of ultrafast lasers, the Chameleon Compact OPO family offers unique benefits. The non-linear medium is a fan-poled crystal that allows simple and broad tunability of the OPO output over a wide range of pump wavelengths. This feature enables truly independent tuning of Chameleon and Compact OPO output wavelengths over hundreds of nanometers. Thanks to the low OPO threshold and high efficiency, 15% of the Chameleon pump beam can be split off and sent directly to the output port to provide simultaneous user access to both the Chameleon and the OPO output beams. This feature enables two-color non- linear microscopy studies with a flexibility that, until now, required at least two independent lasers. The OPO-Vis extends further this flexibility by adding tunability in the UV, visible and near IR wavelengths. Both Chameleon and the Compact OPO families are fully computer-controlled and automatically tuned. Their com- bined output offers an overall tuning range in excess of 900 nm, from 680 nm to 1600 nm, accessible within seconds. This broad and independently accessible tuning Chameleon Ultra II and Compact OPO-Vis Power vs. Wavelength Wavelength (nm) Typical Laser Power (mW) 0 300 10,000 10 100 1000 40050060070080090010001100120013001400150016001750275037504750 Chameleon SHG Chameleon OPO SHG Chameleon Ultra II Chameleon OPO Idler Chameleon OPO Signal Oscillator Accessories37 coherent.com KEY ADVANTAGES • Wavelength extension to IR (OPO) and UV/Visible (OPO-Vis) • Sealed hands-free performance • Designed to be pumped by Chameleon Ultra and Vision families • Automated wavelength tuning with single optic set • Fan-poled OPO crystal for flexible pump wavelength • Independent pump laser (Chameleon) and OPO/OPO SHG tuning • Enables full multimodal imaging (MPE, SHG, THG and CARS) • RS-232 and USB interface • Filtered and dry air recirculation unit to tune across water absorption bands • Vis and Idler outputs available also as after-sale upgrades Ultrafast Laser Systems Chameleon Compact OPO and OPO-Vis range is useful for two-color MPE microscopy, SHG and THG microscopy, and CARS microscopy of lipids. Wavelengths above 1.1 micron optimally excite the new mFruit family of fluorescent proteins at their excitation peaks, in wavelength regions of minimum damage and deepest tissue penetra- tion. The combination of the low pump threshold and the industry-record high power of Chameleon guarantee plenty of power at both the Chameleon and OPO outputs to compensate for losses in complex microscope systems and to enable deep multimodal imaging. The industry’s smallest footprint of Chameleon Compact OPO makes it simple to add this laser to existing multiphoton set-ups and upgrade MPE facilities to fully multimodal operations ready for the most advanced spectral imaging and manipu- lation techniques. Extension into UV and visible wavelengths enables the Chameleon Compact OPO-Vis to address applications beyond multiphoton microscopy, such as spectroscopy and non-linear optics. The optional idler output enables a unique automated tuning range between 340 nm and 1600 nm and 1750 nm to 4000 nm, enabling also time- resolved vibrational spectroscopy. Multi-user facilities will greatly benefit from this unprecedented flexibility while individual investigators will appreciate how the many possible upgrades can follow their changing research objectives, even with limited initial budgets. Chameleon Compact OPO-Vis Oscillator AccessoriesNext >